An ultra-high pressure liquid chromatography-tandem mass spectrometry method for the quantification of teicoplanin in plasma of neonates.
| Τίτλος | An ultra-high pressure liquid chromatography-tandem mass spectrometry method for the quantification of teicoplanin in plasma of neonates. |
| Publication Type | Journal Article |
| Year of Publication | 2017 |
| Authors | Begou, O., Kontou A., Raikos N., Sarafidis K., Roilides E., Papadoyannis I. N., & Gika H. G. |
| Journal | J Chromatogr B Analyt Technol Biomed Life Sci |
| Volume | 1047 |
| Pagination | 215-222 |
| Date Published | 2017 Mar 15 |
| ISSN | 1873-376X |
| Λέξεις κλειδιά | Anti-Bacterial Agents, Chromatography, High Pressure Liquid, Drug Monitoring, Humans, Infant, Newborn, Limit of Detection, Tandem Mass Spectrometry, Teicoplanin |
| Abstract | The development and validation of an ultra-high pressure liquid chromatography (UHPLC) tandem mass spectrometry (MS/MS) method was performed with the aim to be applied for the quantification of plasma teicoplanin concentrations in neonates. Pharmacokinetic data of teicoplanin in the neonatal population is very limited, therefore, a sensitive and reliable method for the determination of all isoforms of teicoplanin applied in a low volume of sample is of real importance. Teicoplanin main components were extracted by a simple acetonitrile precipitation step and analysed on a C18 chromatographic column by a triple quadrupole MS with electrospray ionization. The method provides quantitative data over a linear range of 25-6400ng/mL with LOD 8.5ng/mL and LOQ 25ng/mL for total teicoplanin. The method was applied in plasma samples from neonates to support pharmacokinetic data and proved to be a reliable and fast method for the quantification of teicoplanin concentration levels in plasma of infants during therapy in Intensive Care Unit. |
| DOI | 10.1016/j.jchromb.2016.01.042 |
| Alternate Journal | J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. |
| PubMed ID | 26896312 |
