Δημοσίευση

Using CRISPR-Cas9 to Study ERK Signaling in Drosophila.

ΤίτλοςUsing CRISPR-Cas9 to Study ERK Signaling in Drosophila.
Publication TypeJournal Article
Year of Publication2017
AuthorsForés, M., Papagianni A., Rodríguez-Muñoz L., & Jiménez G.
JournalMethods Mol Biol
Volume1487
Pagination353-365
Date Published2017
ISSN1940-6029
Λέξεις κλειδιάAnimals, Base Sequence, Binding Sites, Clustered Regularly Interspaced Short Palindromic Repeats, CRISPR-Cas Systems, DNA Damage, DNA Repair, Drosophila, MAP Kinase Signaling System, Mutation, Nucleotide Motifs, Phenotype, Protein Binding, Receptor Protein-Tyrosine Kinases, RNA, Guide
Abstract

Genome engineering using the clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR associated nuclease 9 (Cas9) technology is revolutionizing biomedical research. CRISPR-Cas9 enables precise editing of genes in a wide variety of cells and organisms, thereby accelerating molecular studies via targeted mutagenesis, epitope tagging, and other custom genetic modifications. Here, we illustrate the CRISPR-Cas9 methodology by focusing on Capicua (Cic), a nuclear transcriptional repressor directly phosphorylated and inactivated by ERK/MAPK. Specifically, we use CRISPR-Cas9 for targeting an ERK docking site of Drosophila Cic, thus generating ERK-insensitive mutants of this important signaling sensor.

DOI10.1007/978-1-4939-6424-6_26
Alternate JournalMethods Mol. Biol.
PubMed ID27924580

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