Using CRISPR-Cas9 to Study ERK Signaling in Drosophila.
Τίτλος | Using CRISPR-Cas9 to Study ERK Signaling in Drosophila. |
Publication Type | Journal Article |
Year of Publication | 2017 |
Authors | Forés, M., Papagianni A., Rodríguez-Muñoz L., & Jiménez G. |
Journal | Methods Mol Biol |
Volume | 1487 |
Pagination | 353-365 |
Date Published | 2017 |
ISSN | 1940-6029 |
Λέξεις κλειδιά | Animals, Base Sequence, Binding Sites, Clustered Regularly Interspaced Short Palindromic Repeats, CRISPR-Cas Systems, DNA Damage, DNA Repair, Drosophila, MAP Kinase Signaling System, Mutation, Nucleotide Motifs, Phenotype, Protein Binding, Receptor Protein-Tyrosine Kinases, RNA, Guide |
Abstract | Genome engineering using the clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR associated nuclease 9 (Cas9) technology is revolutionizing biomedical research. CRISPR-Cas9 enables precise editing of genes in a wide variety of cells and organisms, thereby accelerating molecular studies via targeted mutagenesis, epitope tagging, and other custom genetic modifications. Here, we illustrate the CRISPR-Cas9 methodology by focusing on Capicua (Cic), a nuclear transcriptional repressor directly phosphorylated and inactivated by ERK/MAPK. Specifically, we use CRISPR-Cas9 for targeting an ERK docking site of Drosophila Cic, thus generating ERK-insensitive mutants of this important signaling sensor. |
DOI | 10.1007/978-1-4939-6424-6_26 |
Alternate Journal | Methods Mol. Biol. |
PubMed ID | 27924580 |