Δημοσίευση

Assessing the axonal translocation of CeO2 and SiO2 nanoparticles in the sciatic nerve fibers of the frog: an ex vivo electrophysiological study.

ΤίτλοςAssessing the axonal translocation of CeO2 and SiO2 nanoparticles in the sciatic nerve fibers of the frog: an ex vivo electrophysiological study.
Publication TypeJournal Article
Year of Publication2015
AuthorsKastrinaki, G., Samsouris C., Kosmidis E. K., Papaioannou E., Konstandopoulos A. G., & Theophilidis G.
JournalInt J Nanomedicine
Volume10
Pagination7089-96
Date Published2015
ISSN1178-2013
Λέξεις κλειδιάAnimals, Anura, Axons, Cerium, Electrophysiology, Female, Male, Nanoparticles, Nerve Fibers, Sciatic Nerve, Silicon Dioxide, Spectroscopy, Fourier Transform Infrared
Abstract

The axonal translocation of two commonly used nanoparticles in medicine, namely CeO2 and SiO2, is investigated. The study was conducted on frog sciatic nerve fibers in an ex vivo preparation. Nanoparticles were applied at the proximal end of the excised nerve. A nerve stimulation protocol was followed for over 35 hours. Nerve vitality curve comparison between control and exposed nerves showed that CeO2 has no neurotoxic effect at the concentrations tested. After exposure, specimens were fixed and then screen scanned every 1 mm along their length for nanoparticle presence by means of Fourier transform infrared microscopy. We demonstrated that both nanoparticles translocate within the nerve by formation of narrow bands in the Fourier transform infrared spectrum. For the CeO2, we also demonstrated that the translocation depends on both axonal integrity and electrical activity. The speed of translocation for the two species was estimated in the range of 0.45-0.58 mm/h, close to slow axonal transportation rate. Transmission electron microscopy provided direct evidence for the presence of SiO2 in the treated nerves.

DOI10.2147/IJN.S93663
Alternate JournalInt J Nanomedicine
PubMed ID26648718
PubMed Central IDPMC4664516

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