Δημοσίευση

Benign hyperplasia of the human prostate is associated with tissue enrichment in chondroitin sulphate of wide size distribution.

ΤίτλοςBenign hyperplasia of the human prostate is associated with tissue enrichment in chondroitin sulphate of wide size distribution.
Publication TypeJournal Article
Year of Publication2000
AuthorsGoulas, A., Hatzichristou D. G., Karakiulakis G., Mirtsou-Fidani V., Kalinderis A., & Papakonstantinou E.
JournalProstate
Volume44
Issue2
Pagination104-10
Date Published2000 Jul 01
ISSN0270-4137
Λέξεις κλειδιάAdult, beta-Galactosidase, Chondroitin ABC Lyase, Chondroitin Lyases, Chondroitin Sulfates, Chromatography, Ion Exchange, Dermatan Sulfate, Electrophoresis, Cellulose Acetate, Electrophoresis, Polyacrylamide Gel, Extracellular Matrix, Glycosaminoglycans, Glycoside Hydrolases, Heparitin Sulfate, Humans, Hyaluronic Acid, Hyaluronoglucosaminidase, Male, Polysaccharide-Lyases, Prostatic Hyperplasia
Abstract

BACKGROUND: Benign prostatic hyperplasia (BPH) involves qualitative and quantitative alterations in extracellular matrix (ECM) components affecting stromal-epithelial interactions. Glycosaminoglycans (GAGs) are polysaccharide components of the ECM whose role in the development of BPH is under investigation.
METHODS: GAGs were extracted from human prostates of normal and BPH origin and were subsequently fractionated through DEAE-sephacel anion exchange chromatography. The isolated GAG fractions were identified through electrophoresis on cellulose acetate membranes and treatment with GAG-degrading enzymes of known specificity. Their size distribution was determined through gradient polyacrylamide gel electrophoresis.
RESULTS: Isolated prostatic GAGs included hyaluronic acid (HA), heparan sulphate (HS), and a mixture of dermatan sulphate (DS) and chondroitin sulphate (CS). The CS/DS ratio was significantly higher in hyperplastic as compared to normal prostates. A difference was also observed with respect to the apparent molecular mass of the DS-CS mixture, which reflects the CS enrichment in BPH. GAGs isolated from hyperplastic prostates were more diverse in size as compared to the corresponding glycans from normal prostates.
CONCLUSIONS: The apparent increase in CS and decrease in DS content in prostates of patients with BPH is in good agreement with the pathological manifestation of increased cell proliferation in hyperplastic prostate tissue, since these glycan molecules have been reported to increase and decrease cell proliferation, respectively. Identification of the responsible enzymes involved in the homeostasis of CS and DS may provide alternative targets for pharmacological intervention.

DOI10.1002/1097-0045(20000701)44:2<104::aid-pros2>3.0.co;2-6
Alternate JournalProstate
PubMed ID10881019

Επικοινωνία

Τμήμα Ιατρικής, Πανεπιστημιούπολη ΑΠΘ, T.K. 54124, Θεσσαλονίκη
 

Συνδεθείτε

Το τμήμα Ιατρικής στα κοινωνικά δίκτυα.
Ακολουθήστε μας ή συνδεθείτε μαζί μας.