Delivery of hepatocyte growth factor mRNA from nanofibrillar scaffolds in a pig model of peripheral arterial disease.
Τίτλος | Delivery of hepatocyte growth factor mRNA from nanofibrillar scaffolds in a pig model of peripheral arterial disease. |
Publication Type | Journal Article |
Year of Publication | 2020 |
Authors | Zaitseva, T. S., Yang G., Dionyssiou D., Zamani M., Sawamura S., Yakubov E., Ferguson J., Hallett R. L., Fleischmann D., Paukshto M. V., & Huang N. F. |
Journal | Regen Med |
Volume | 15 |
Issue | 6 |
Pagination | 1761-1773 |
Date Published | 2020 06 |
ISSN | 1746-076X |
Abstract | Chemical modification of mRNA (mmRNA) substantially improves their stability and translational efficiency within cells. Nanofibrillar collagen scaffolds were previously shown to enable the spatially localized delivery and temporally controlled release of mmRNA encoding HGF both and . Herein we developed an improved slow-releasing HGF mmRNA scaffold and tested its therapeutic efficacy in a porcine model of peripheral arterial disease. The HGF mmRNA was released from scaffolds in a temporally controlled fashion with preserved transfection activity. The mmRNA scaffolds improved vascular regeneration when sutured to the ligated porcine femoral artery. These studies validate the therapeutic potential of HGF mmRNA delivery from nanofibrillar scaffolds for treatment of peripheral arterial disease. |
DOI | 10.2217/rme-2020-0023 |
Alternate Journal | Regen Med |
PubMed ID | 32772903 |
Grant List | R01 HL127113 / HL / NHLBI NIH HHS / United States R01 HL142718 / HL / NHLBI NIH HHS / United States |