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Development and validation of a HILIC- MS/MS multi-targeted method for metabolomics applications.

ΤίτλοςDevelopment and validation of a HILIC- MS/MS multi-targeted method for metabolomics applications.
Publication TypeJournal Article
Year of Publication2015
AuthorsVirgiliou, C., Sampsonidis I., Gika H. G., Raikos N., & Theodoridis G. A.
JournalElectrophoresis
Date Published2015 Jul 14
ISSN1522-2683
Abstract

The paper reports the development of a multi-analyte method and its application in metabolic profiling of biological fluids. The initial aim of the method was the quantification of metabolites existing in cell culture medium used in in-vitro fertilisation (IVF) and in other biological fluids related to embryo growth. Since most of these analytes are polar primary metabolites a hydrophilic interaction liquid chromatography (HILIC) system was selected. The analytical system comprised Ultra (High) performance liquid chromatography (UHPLC) with detection on a triple quadrupole mass spectrometer operating in both positive and negative modes. Mobile phase and gradient elution conditions were studied with the aim to achieve the highest coverage of metabolic space in a single injection namely the largest number of analytes that could be detected and quantified. The developed method provides absolute quantitation of ca. 100 metabolites belonging to key metabolite classes such as sugars, aminoacids, nucleotides, organic acids and amines. Following validation, the method was applied for the metabolic profiling of hundreds of samples of spent culture medium originating from human IVF procedures and several hundreds of biological samples such as amniotic fluid, human urine and blood serum from pregnant women. The bioanalytical end-point was to provide assistance in the process of embryo transfer and improving IVF success rates but also to provide insight in complications related to the subsequent embryo growth during pregnancy. This article is protected by copyright. All rights reserved.

DOI10.1002/elps.201500208
Alternate JournalElectrophoresis
PubMed ID26180020

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