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Differentiation Capacity of Monocyte-Derived Multipotential Cells on Nanocomposite Poly(e-caprolactone)-Based Thin Films.

ΤίτλοςDifferentiation Capacity of Monocyte-Derived Multipotential Cells on Nanocomposite Poly(e-caprolactone)-Based Thin Films.
Publication TypeJournal Article
Year of Publication2019
AuthorsKoliakou, I., Gounari E., Nerantzaki M., Pavlidou E., Bikiaris D., Kaloyianni M., & Koliakos G.
JournalTissue Eng Regen Med
Volume16
Issue2
Pagination161-175
Date Published2019 04
ISSN2212-5469
Λέξεις κλειδιάBiomarkers, Cell Differentiation, Cells, Cultured, Human Umbilical Vein Endothelial Cells, Humans, Hydroxyapatites, Membranes, Artificial, Mesenchymal Stem Cells, Monocytes, Multipotent Stem Cells, Nanocomposites, Nanotubes, Osteoblasts, Polyesters, Silicon Dioxide, Strontium, Tissue Engineering, Tissue Scaffolds, Transcription, Genetic, Wharton Jelly
Abstract

Background: Μonocyte-derived multipotential cells (MOMCs) include progenitors capable of differentiation into multiple cell lineages and thus represent an ideal autologous transplantable cell source for regenerative medicine. In this study, we cultured MOMCs, generated from mononuclear cells of peripheral blood, on the surface of nanocomposite thin films.
Methods: For this purpose, nanocomposite Poly(e-caprolactone) (PCL)-based thin films containing either 2.5 wt% silica nanotubes (SiOntbs) or strontium hydroxyapatite nanorods (SrHAnrds), were prepared using the spin-coating method. The induced differentiation capacity of MOMCs, towards bone and endothelium, was estimated using flow cytometry, real-time polymerase chain reaction, scanning electron microscopy and fluorescence microscopy after cells' genetic modification using the Sleeping Beauty Transposon System aiming their observation onto the scaffolds. Moreover, Wharton's Jelly Mesenchymal Stromal Cells were cultivated as a control cell line, while Human Umbilical Vein Endothelial Cells were used to strengthen and accelerate the differentiation procedure in semi-permeable culture systems. Finally, the cytotoxicity of the studied materials was checked with MTT assay.
Results: The highest differentiation capacity of MOMCs was observed on PCL/SiOntbs 2.5 wt% nanocomposite film, as they progressively lost their native markers and gained endothelial lineage, in both protein and transcriptional level. In addition, the presence of SrHAnrds in the PCL matrix triggered processes related to osteoblast bone formation.
Conclusion: To conclude, the differentiation of MOMCs was selectively guided by incorporating SiOntbs or SrHAnrds into a polymeric matrix, for the first time.

DOI10.1007/s13770-019-00185-z
Alternate JournalTissue Eng Regen Med
PubMed ID30989043
PubMed Central IDPMC6439045

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