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The Effect of Low Intensity Extracorporeal Shockwave Treatment on the Urinary Bladder of an Experimental Diabetic Rat Model.

ΤίτλοςThe Effect of Low Intensity Extracorporeal Shockwave Treatment on the Urinary Bladder of an Experimental Diabetic Rat Model.
Publication TypeJournal Article
Year of Publication2021
AuthorsDimitriadis, F., Papaioannou M., Sokolakis I., Fragou A., Hatzichristou D., & Apostolidis A.
JournalInt Neurourol J
Date Published2021 Mar 06
ISSN2093-4777
Abstract

Purpose: Increasing preclinical data support an impact of low intensity extracorporeal shockwave therapy (Li-ESWT) on the bladder. We investigated the molecular effect of LiESWT on the bladder of a streptozotocin induced diabetic rat model.
Methods: Fifteen 8-week old male Wistar rats were randomized into 3 groups: a control group (Control bladder CB; n=5), a group of diabetic rats without treatment (diabetes mellitus DM, n=5) and a group of diabetic rats treated with Li-ESWT (DM-ESWT; n=5). For the induction of diabetes, a single intraperitoneal dose of streptozotocin (60mg/kg) was used. Twenty days after the induction of diabetes, each rat in group DM-ESWT received 300 shockwaves with an energy flux density of 0.09 mJ/mm2. Sessions were repeated three times/week for two weeks, followed by a two-week washout period. Total RNA from bladder tissue was extracted, cDNA was synthesized and Quantitative Real Time Polymerase Chain Reaction was performed to analyze the expression pattern of the Transient Receptor Potential Vanilloid 1 (TRPV1), interleukin 1β (IL1b) and the muscarinic receptors M1, M2, and M3 (Chrm1, Chrm2, and Chrm3).
Results: The expression of TRPV1, IL1b, and Chrm2 genes was significantly different between the three groups (p=0.002, <0.0001 and 0.011, respectively) (one-way ANOVA). In the DM group, the expression of all genes was increased in comparison with the control group, but statistical significance was observed only for TRPV1 and IL1b genes (p=0.002 and <0.0001, respectively). Li-ESWT significantly reduced the expression of IL1b and Chrm2 (p=0.001 and 0.011, respectively), whereas a tendency for reduced expression was noted for TRPV1 (p=0.069).
Conclusions: The induction of diabetes mellitus was associated with increased expression of genes related to mechanosensation, inflammation/ischemia, and contraction in the rat bladder. Li-ESWT reduced the expression of IL1b, Chrm2 and lesser of TRPV1 receptor genes towards controls' levels, suggesting a therapeutic potential of this treatment modality for diabetic cystopathy.

DOI10.5213/inj.2040344.172
Alternate JournalInt Neurourol J
PubMed ID33676379

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