Δημοσίευση

The effects of chronically increased intra-abdominal pressure on the rabbit diaphragm.

ΤίτλοςThe effects of chronically increased intra-abdominal pressure on the rabbit diaphragm.
Publication TypeJournal Article
Year of Publication2012
AuthorsPapavramidis, T. S., Kotidis E., Ioannidis K., Cheva A., Lazou T., Koliakos G., Karkavelas G., & Papavramidis S. T.
JournalObes Surg
Volume22
Issue3
Pagination487-92
Date Published2012 Mar
ISSN1708-0428
Λέξεις κλειδιάAbdominal Muscles, Adenosine Triphosphatases, Animals, Diaphragm, Disease Models, Animal, Glutathione Peroxidase, Glutathione Reductase, Intra-Abdominal Hypertension, Lipid Peroxidation, Male, Muscle Fibers, Fast-Twitch, Muscle Fibers, Slow-Twitch, Oxidative Stress, Pressure, Protein Carbonylation, Rabbits
Abstract

BACKGROUND: Diaphragmatic muscular remodeling is caused by various conditions and was mainly studied in pulmonary pathologies and chronic alterations of intra-thoracic pressure. We investigate the effect of the chronically increased intra-abdominal pressure (IAP) on the diaphragm by morphological and biochemical analysis.METHODS: Thirty rabbits were divided into control and study groups. IAP was increased in group B to 12 mmHg for 2 months. The left hemidiaphragm underwent morphological, while the right underwent biochemical analysis.RESULTS: In H&E, all fibers were normal. ATPase analysis demonstrated that type I fibers show no differences between groups. Type ΙΙ(Α) were decreased (p = 0.016) while type ΙΙ(Β/X) fibers were increased (p = 0.025) in group B. Fibers with resistance to fatigue were decreased in group B (p = 0.024). In group B, biochemical activity for glutathione reductase (p = 0.004), glutathione peroxidase (p = 0.021), protein carbonylation (0.029), lipid peroxidation (p = 0.005), and balance of preoxidative-antioxidative factors (p = 0.006) was increased.CONCLUSIONS: Chronically increased IAP induces alterations to the rabbit diaphragm. Adaptation, equivalent to strenuous contraction, transforms the diaphragm to be functionally more efficient toward workload but makes it vulnerable against oxidative stress.

DOI10.1007/s11695-012-0587-2
Alternate JournalObes Surg
PubMed ID22246392

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