Background: Identifying combinations of the hip and knee joint angles which can selectively recruit specific hamstring muscles may be beneficial for injury prevention or rehabilitation. The purpose of this study was to examine the joint torque and electromyographic (EMG) activity of the semitendinosus (ST) and biceps femoris long head (BFlh).Methods: Twenty subjects performed maximum isokinetic concentric and eccentric knee flexor efforts at 60°·s1, 120°·s1 and 150°·s1 from three different hip joint angles while surface EMG of ST and BFlh was recorded.Results: Analysis of variance showed that there are no inter-muscular differences in EMG amplitude across testing conditions (p > .05). Peak EMG occurred near full knee extension for the BFLh and at a higher flexion angle for the ST while exercise from a prone position shifted the peak EMG towards higher knee flexion angle (p < 0.05).Conclusion: Maximal dynamic knee flexion exercises do not induce a higher EMG amplitude of BFlh or ST. Exercising from a higher hip flexion angle near full knee extension may selectively activate the BFlh.Level of evidence: IIb.