Δημοσίευση

Effects of Wnt-1 blockade in DEN-induced hepatocellular adenomas of mice.

ΤίτλοςEffects of Wnt-1 blockade in DEN-induced hepatocellular adenomas of mice.
Publication TypeJournal Article
Year of Publication2018
AuthorsSklavos, A., Poutahidis T., Giakoustidis A., Makedou K., Angelopoulou K., Hardas A., Andreani P., Zacharioudaki A., Saridis G., Goulopoulos T., Tsarea K., Karamperi M., Papadopoulos V., Papanikolaou V., Papalois A., Iliadis S., Mudan S., Azoulay D., & Giakoustidis D.
JournalOncol Lett
Volume15
Issue1
Pagination1211-1219
Date Published2018 Jan
ISSN1792-1074
Abstract

Recent evidence has suggested that downregulation of the Wnt/β-catenin signaling pathway may contribute to the development and growth of HCC. Consequently, elements of this pathway have begun to emerge as potential targets for improving outcomes of anti-HCC. Thus, the present study sought to examine the effects of Wnt-1 blockade using the classical diethylnitrosamine (DEN)-induced chemical carcinogenesis mouse model of HCC. The depletion of Wnt-1 using neutralizing antisera was done for ten consecutive days at the age of 9 months and mice were examined for the following 20 days. At that time, DEN-treated mice had multiple variably-sized hepatic cell adenomas. Anti-Wnt-1 was particularly potent in suppressing the expression of critical elements of the Wnt/β-catenin signaling pathway, such as β-catenin and Frizzled-1 receptor, however, not Dickkopf-related protein 1. This effect co-existed with the suppression of Cyclin D1, FOXM1, NF-κΒ and c-Jun commensurate with proliferation and apoptosis blockade in hepatocellular adenomas, and reduced Bcl-2 and c-Met in the serum of mice. Nonetheless, tumor size and multiplicity were found to be unaffected, suggesting that apoptosis may be equally important to proliferation in the context of counteracting DEN induced hepatocellular adenomas of mice.

DOI10.3892/ol.2017.7427
Alternate JournalOncol Lett
PubMed ID29399175
PubMed Central IDPMC5772790

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