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Enhanced deposition of low-molecular-weight hyaluronan in lungs of cigarette smoke-exposed mice.

ΤίτλοςEnhanced deposition of low-molecular-weight hyaluronan in lungs of cigarette smoke-exposed mice.
Publication TypeJournal Article
Year of Publication2010
AuthorsBracke, K. R., Dentener M. A., Papakonstantinou E., Vernooy J. H. J., Demoor T., Pauwels N. S., Cleutjens J., van Suylen R. Jan, Joos G. F., Brusselle G. G., & Wouters E. F. M.
JournalAm J Respir Cell Mol Biol
Volume42
Issue6
Pagination753-61
Date Published2010 Jun
ISSN1535-4989
Λέξεις κλειδιάAirway Remodeling, Animals, Bronchi, Bronchoalveolar Lavage Fluid, Collagen, Disease Models, Animal, Fibronectins, Gene Expression Regulation, Enzymologic, Glucuronosyltransferase, Hyaluronic Acid, Hyaluronoglucosaminidase, Macrophages, Alveolar, Male, Mice, Mice, Inbred C57BL, Molecular Weight, Pulmonary Alveoli, Pulmonary Disease, Chronic Obstructive, Pulmonary Emphysema, RNA, Messenger, Smoking, Time Factors
Abstract

Chronic obstructive pulmonary disease (COPD) is characterized by infiltration of inflammatory cells, destruction of lung parenchyma, and airway wall remodeling. Hyaluronan (HA) is a component of the extracellular matrix, and low-molecular-weight (LMW) HA fragments have proinflammatory capacities. We evaluated the presence of HA in alveolar and airway walls of C57BL/6 mice that were exposed to air or cigarette smoke (CS) for 4 weeks (subacute) or 24 weeks (chronic). We measured deposition of the extracellular matrix proteins collagen and fibronectin in airway walls and determined the molecular weight of HA purified from lung tissue. In addition, we studied the expression of HA-modulating genes by RT-PCR. HA staining in alveolar walls was significantly enhanced upon chronic CS exposure, whereas HA levels in the airway walls were already significantly higher upon subacute CS exposure and remained elevated upon chronic CS exposure. This differed from the deposition of collagen and fibronectin, which are only elevated at the chronic time point. In lungs of CS-exposed mice, the molecular weight of HA clearly shifted toward more LMW HA fragments. CS exposure significantly increased the mRNA expression of the HA synthase gene Has3 in total lung tissue, whereas the expression of Has1 was decreased. These in vivo studies in an experimental model of COPD show that CS exposure leads to enhanced deposition of (mostly LMW) HA in alveolar and bronchial walls by altering the expression of HA-modulating enzymes. This may contribute to airway wall remodeling and pulmonary inflammation in COPD.

DOI10.1165/rcmb.2008-0424OC
Alternate JournalAm. J. Respir. Cell Mol. Biol.
PubMed ID19675307

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