Interleukin-6 and the matrix metalloproteinase response in the vitreous during proliferative vitreoretinopathy.
Τίτλος | Interleukin-6 and the matrix metalloproteinase response in the vitreous during proliferative vitreoretinopathy. |
Publication Type | Journal Article |
Year of Publication | 2011 |
Authors | Symeonidis, C., Papakonstantinou E., Androudi S., Rotsos T., Diza E., Brazitikos P., Karakiulakis G., & Dimitrakos S. A. |
Journal | Cytokine |
Volume | 54 |
Issue | 2 |
Pagination | 212-7 |
Date Published | 2011 May |
ISSN | 1096-0023 |
Λέξεις κλειδιά | Adult, Aged, Aged, 80 and over, Enzyme-Linked Immunosorbent Assay, Female, Humans, Interleukin-6, Male, Matrix Metalloproteinases, Middle Aged, Vitreoretinopathy, Proliferative, Vitreous Body |
Abstract | PURPOSE: To investigate the levels of IL-6 in the vitreous of patients with RRD complicated with PVR and correlate the IL-6 levels with matrix metalloproteinase (MMP)-1,-2,-3,-8,-9 and tissue inhibitor of metalloproteinases (TIMP)-1 with respect to RRD extent, duration and PVR grade.DESIGN: Cohort study.PARTICIPANTS: Twenty-eight vitreous samples from 28 eyes of 28 patients with RRD complicated with PVR.METHODS: Institutional study. Twenty-eight vitreous samples from 28 eyes of 28 patients with RRD complicated with PVR were collected during pars plana vitrectomy (PPV) and were compared to vitreous control samples. IL-6, MMP-1,-3,-8 and TIMP-1 levels were measured using ELISA while enzymatic activity of MMP-2, and -9 was determined employing gelatin zymography.RESULTS: Protein IL-6 (p=0.030), MMP-1 (p=0.003), MMP-3 (p=0.003), TIMP-1 (p=0.001) levels as well as enzymatic activity of proMMP-9 (p=0.013), MMP-9 (p=0.017) and proMMP-2 (p=0.010), were significantly increased in PVR patients as compared to controls. IL-6 levels correlated with MMP-1 (p=0.002), proMMP-2 (p=0.006), MMP-3 (p=0.001) and TIMP-1 (p=0.006). Regression analysis revealed positive correlations between IL-6 and all MMPs and TIMP-1.CONCLUSIONS: Taking into account the previously established effect of interleukins in MMP activity, the findings of this study suggest a role of IL-6 in MMP stimulation during PVR development. |
DOI | 10.1016/j.cyto.2011.02.001 |
Alternate Journal | Cytokine |
PubMed ID | 21353595 |