Tumor Infiltrating Lymphocytes and CD8+ T Cell Subsets as Prognostic Markers in Patients with Surgically Treated Laryngeal Squamous Cell Carcinoma.
Τίτλος | Tumor Infiltrating Lymphocytes and CD8+ T Cell Subsets as Prognostic Markers in Patients with Surgically Treated Laryngeal Squamous Cell Carcinoma. |
Publication Type | Journal Article |
Year of Publication | 2020 |
Authors | Chatzopoulos, K., Kotoula V., Manoussou K., Markou K., Vlachtsis K., Angouridakis N., Nikolaou A., Vassilakopoulou M., Psyrri A., & Fountzilas G. |
Journal | Head Neck Pathol |
Volume | 14 |
Issue | 3 |
Pagination | 689-700 |
Date Published | 2020 Sep |
ISSN | 1936-0568 |
Abstract | To evaluate the prognostic significance of tumor infiltrating lymphocytes (TILs) and of CD8+ T-cell subsets in patients with surgically treated laryngeal squamous cell carcinoma (LSCC), LSCC from 283 patients were examined. TIL density was morphologically assessed on whole sections. CD8+ cell counts/mm were evaluated on multiple tissue microarray cores per tumor (median counts for high/low CD8+/mm). TIL density and CD8+ counts weakly correlated with each other (Spearman's rho = 0.348). Heterogeneous CD8+ counts/mm were demonstrated in 28% of the tumors. In univariate analysis, a significant interaction was observed between CD8 expression and nodal status with respect to outcome; in node-positive patients, those with high CD8+ tumors had 77% lower risk of relapse (interaction p < 0.001) and 74% lower risk for death (interaction p = 0.002) compared to patients with low CD8+ tumors. In multivariate analysis, higher TIL density independently conferred lower risk for relapse in the entire cohort (HR 0.87; 95% CI 0.77-0.98; Wald's p = 0.017) and in node-positive patients (HR 0.41; 95% CI 0.23-0.75; p = 0.003) and, similarly, for death (p = 0.025 and p = 0.003, respectively). High CD8+ was not a significant independent prognostic marker in any analysis setting. The assessment of CD8+ infiltrates does not seem to offer additional prognostic information over the morphologically assessed TIL density. It also appears that the favorable prognostic impact of higher TIL density and CD8+ infiltrates mostly concerns node-positive but not node-negative disease. If validated in larger node-positive cohorts, these findings are worth considering for the diagnostic development of immune cell infiltrates in LSCC. |
DOI | 10.1007/s12105-019-01101-6 |
Alternate Journal | Head Neck Pathol |
PubMed ID | 31749124 |
PubMed Central ID | PMC7413976 |
Grant List | Research Grant HE R_5G / / Ellenike Synergazomene Onkologike Omada / |