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A two-step approach improves the diagnosis of Clostridium difficile infection.

TitleA two-step approach improves the diagnosis of Clostridium difficile infection.
Publication TypeJournal Article
Year of Publication2017
AuthorsKachrimanidou, M., Tegou Z., Chasampalioti M., Arvaniti K., Protonotariou E., & Skoura L.
JournalJ Microbiol Methods
Date Published2017 Dec
KeywordsBacterial Proteins, Bacterial Toxins, Clostridium Infections, DNA, Bacterial, Enterotoxins, Glutamate Dehydrogenase, Humans, Immunoenzyme Techniques, Polymerase Chain Reaction, Sensitivity and Specificity

Clostridium difficile infection (CDI) is a major cause of health care-associated diarrhea. The aim of the present study was to evaluate a two-step approach for the diagnosis of CDI. The two-step procedure consisted of GDH-toxin A/B EIA (Enzyme immunoassay targeting enterotoxin A and Cytotoxin B), followed by PCR detecting toxigenic C. difficile. Results indicate that EIAs provide a rapid screening assay for the laboratory diagnosis of CDI but, in GDH-positive and toxins-negative samples, EIA should be always followed by PCR to distinguish toxigenic vs nontoxigenic strains. GDH-toxin A/B EIA-rapid test has high specificity but low sensitivity to detect CDI. The implementation of a two-step procedure significantly increases the diagnostic accuracy to detect CDI and provides a toxigenic type characterization of C. difficile isolates.

Alternate JournalJ. Microbiol. Methods
PubMed ID28970055


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