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Targeting hepatocarcinogenesis model in C56BL6 mice with pan-aurora kinase inhibitor Danusertib.

TitleTargeting hepatocarcinogenesis model in C56BL6 mice with pan-aurora kinase inhibitor Danusertib.
Publication TypeJournal Article
Year of Publication2018
AuthorsGavriilidis, P., Poutahidis T., Giakoustidis A., Makedou K., Angelopoulou K., Hardas A., Andreani P., Zacharioudaki A., Saridis G., Gargavanis A., Louri E., Antoniadis N., Karampela E., Psychalakis N., Michalopoulos A., Papalois A., Iliadis S., Mudan S., Azoulay D., & Giakoustidis D.
JournalJ Cancer
Date Published2018

: To elucidate the expression of Aurora kinases (AURK) and the anticancer effects of pan-aurora kinase inhibitor Danusertib in hepatocarcinogenesis model in C56Bl6 mice. : Thirty mice C56Bl6 were randomly divided into Group A or control, Group B animals who underwent experimental hepatocarcinogenesis with diethylnitrosamine (DEN), and Group C animals with DEN-induced hepatocarcinogenenesis that treated with pan-aurora kinase inhibitor Danusertib. Primary antibodies for immunochistochemistry (IHC) included rabbit antibodies against Ki-67, DKK1, INCENP, cleaved caspase-3, NF-κB p65, c-Jun, β-catenin. Hepatocyte growth factor receptor (C-MET/HGFR) and Bcl-2 antagonist of cell death (BAD) serum levels were determined using a quantitative sandwich enzyme immunoassay technique. : Inhibition of AURK reduced the number of DEN-induced liver tumours. Apoptosis and proliferation was very low in both DEN-induced and anti- AURK groups respectively. The hepatocellular adenoma cells of DEN-treated mice uniformly had ample nuclear INCENP whereas in anti- AURK markedly decreased. Expression of β-catenin, NF-kB and c-Jun did not differ in liver tumors of both AURK -depleted and non-depleted mice. Depletion of AURK reduced the number of DEN-induced hepatic tumours. However, their size did not differ significantly between the groups.

Alternate JournalJ Cancer
PubMed ID29581770
PubMed Central IDPMC5868156


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