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Cyclin D1 overexpression in multiple myeloma. A morphologic, immunohistochemical, and in situ hybridization study of 71 paraffin-embedded bone marrow biopsy specimens.

TitleCyclin D1 overexpression in multiple myeloma. A morphologic, immunohistochemical, and in situ hybridization study of 71 paraffin-embedded bone marrow biopsy specimens.
Publication TypeJournal Article
Year of Publication2001
AuthorsAthanasiou, E., Kaloutsi V., Kotoula V., Hytiroglou P., Kostopoulos I., Zervas C., Kalogiannidis P., Fassas A., Christakis J. I., & Papadimitriou C. S.
JournalAm J Clin Pathol
Volume116
Issue4
Pagination535-42
Date Published2001 Oct
ISSN0002-9173
KeywordsAntigens, CD20, Biopsy, Biotinylation, Bone Marrow, Cell Differentiation, Cyclin D1, Female, Gene Expression, Humans, Immunoglobulin kappa-Chains, Immunoglobulin lambda-Chains, Immunohistochemistry, In Situ Hybridization, Male, Middle Aged, Multiple Myeloma, Neoplasm Staging, Paraffin, Plasma Cells, Reverse Transcriptase Polymerase Chain Reaction, RNA, Messenger, Tissue Embedding
Abstract

Cyclin D1 expression was evaluated by immunohistochemical analysis and biotin-labeled in situ hybridization (ISH) in a series of 71 decalcified, paraffin-embedded bone marrow biopsy specimens from patients with multiple myeloma (MM). Cyclin D1 messenger RNA (mRNA) overexpression was detected by ISH in 23 (32%) of 71 cases, whereas cyclin D1 protein was identified by immunohistochemical analysis in 17 (24%) of 71 specimens. All cases that were positive by immunohistochemical analysis also were positive by ISH. Statistically significant associations were found between cyclin D1 overexpression and grade of plasma cell differentiation and between cyclin D1 overexpression and extent of bone marrow infiltration. Our findings demonstrate the following: (1) ISH for cyclin D1 mRNA is a sensitive method for the evaluation of cyclin D1 overexpression in paraffin-embedded bone marrow biopsy specimens with MM. (2) ISH is more sensitive than immunohistochemical analysis in the assessment of cyclin D1 expression. (3) Cyclin D1 overexpression in MM is correlated positively with higher histologic grade and stage.

DOI10.1309/BVT4-YP41-LCV2-5GT0
Alternate JournalAm. J. Clin. Pathol.
PubMed ID11601138

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