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Short communication: Bovine mastitis caused by a multidrug-resistant, mcr-1-positive (colistin-resistant), extended-spectrum β-lactamase-producing Escherichia coli clone on a Greek dairy farm.

TitleShort communication: Bovine mastitis caused by a multidrug-resistant, mcr-1-positive (colistin-resistant), extended-spectrum β-lactamase-producing Escherichia coli clone on a Greek dairy farm.
Publication TypeJournal Article
Year of Publication2020
AuthorsFilioussis, G., Kachrimanidou M., Christodoulopoulos G., Kyritsi M., Hadjichristodoulou C., Adamopoulou M., Tzivara A., Kritas S. K., & Grinberg A.
JournalJ Dairy Sci
Volume103
Issue1
Pagination852-857
Date Published2020 Jan
ISSN1525-3198
KeywordsAnimals, Anti-Bacterial Agents, beta-Lactamases, Cattle, Cephalosporins, Colistin, Dairying, Drug Resistance, Bacterial, Escherichia coli, Escherichia coli Infections, Escherichia coli Proteins, Farms, Female, Greece, Mastitis, Bovine, Microbial Sensitivity Tests, Milk, Multilocus Sequence Typing
Abstract

We performed a survey aimed at analyzing milk samples collected from cows with mastitis for the presence of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli. Single-quarter mastitic milk samples obtained from 400 cows in 23 Greek dairy herds with a history of E. coli mastitis were processed for the selective isolation of ESBL-producing E. coli. The antimicrobial susceptibility of the ESBL-producing isolates was analyzed using agar disk diffusion, and minimum inhibitory concentrations of colistin were determined by broth microdilution. We used PCR followed by DNA sequencing to characterize the β-lactamases and mcr-1 (colistin resistance) genes, and for phylotyping and multilocus sequence typing. We found a total of 89/400 (22.25%) E. coli isolates from 12/23 (52%) farms. Six isolates originating from 6 cows on a single farm were ESBL producers and were resistant to cefquinome, amoxicillin-clavulanic acid, aztreonam, ampicillin, and colistin. Five of these isolates were resistant to trimethoprim-sulfamethoxazole, and 5 to streptomycin. The 6 ESBL producers were mcr-1-positive and carried bla genes; 3 also carried bla genes, and 3 carried bla genes. All of the ESBL producers belonged to phylogroup A, multilocus sequence type ST666 (n = 5), or a single locus variant of ST666 (n = 1). To our knowledge, this is the first report of endemic bovine mastitis caused by mcr-1-positive, ESBL-producing E. coli. These results highlight the value of active surveillance of antimicrobial resistance not commonly tested by diagnostic laboratories for the early detection of novel resistant strains.

DOI10.3168/jds.2019-17320
Alternate JournalJ. Dairy Sci.
PubMed ID31733863

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