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Th2/Th17 cytokine profile in phenotyped Greek asthmatics and relationship to biomarkers of inflammation.

TitleTh2/Th17 cytokine profile in phenotyped Greek asthmatics and relationship to biomarkers of inflammation.
Publication TypeJournal Article
Year of Publication2019
AuthorsDomvri, K., Porpodis K., Tzimagiorgis G., Chatzopoulou F., Kontakiotis T., Kyriazis G., & Papakosta D.
JournalRespir Med
Volume151
Pagination102-110
Date Published2019 05
ISSN1532-3064
KeywordsAsthma, Biomarkers, Case-Control Studies, Eosinophils, Exhalation, Female, Genotype, Greece, Humans, Immunoglobulin E, Interleukins, Leukocyte Count, Male, Middle Aged, Nitric Oxide, Polymorphism, Single Nucleotide, Th17 Cells, Th2 Cells, Transforming Growth Factor beta1
Abstract

OBJECTIVE: The aim of the present study was to investigate the Th2/Th17 pathway in asthmatic patients and also the relationship to asthma severity and biomarkers of inflammation.
METHODS: 90 asthmatic patients, 51 patients with severe, 39 patients with mild asthma and 98 healthy controls were included. Skin prick tests, blood eosinophils, total serum IgE and exhaled FeNO were evaluated. Serum levels of IL-4, IL-5, IL-13, IL-6, IL-17A, IL-23 and TGFβ1 were determined by Flow Cytometry using a panel kit (AimPlex Biosciences). The SNP of IL17A (rs17880588) was genotyped using reverse transcriptase polymerase chain reaction (RT-PCR).
RESULTS: The genotype of the SNP in IL17A (rs 17880588) was similar among all three groups. Serum levels of IL-4, IL-5, IL-13, IL-6, IL-17A and IL-23 were higher in asthmatics compared to controls (p < 0.05). In addition, IL-17A and IL-4 serum levels were found significantly elevated in patients with allergic asthma (p < 0.05). Furthermore, IL-4, IL-5, IL-13 and IL-23 were found significantly higher in patients with eosinophil cut off values above 300 cells/μl (p < 0.05). IL-17A levels were positively correlated with FeNO values in severe asthmatics with eosinophils>400 cells/μl.
CONCLUSIONS: The above findings suggest the coexistence of Th2/Th17 pathway in severe, eosinophilic and in allergic asthma.

DOI10.1016/j.rmed.2019.03.017
Alternate JournalRespir Med
PubMed ID31047104

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