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Potential prognostic value of intracellular cytokine detection by flow cytometry in pulmonary sarcoidosis.

TitlePotential prognostic value of intracellular cytokine detection by flow cytometry in pulmonary sarcoidosis.
Publication TypeJournal Article
Year of Publication2013
AuthorsGounari, E., Chatzizisi O., Diza-Mataftsi E., Papakosta D., Kontakiotis T., Iakovidis D., Zoglopitis F., Bougiouklis D., Markopoulou A., Serasli E., & Kyriazis G.
JournalJ Interferon Cytokine Res
Volume33
Issue5
Pagination261-9
Date Published2013 May
ISSN1557-7465
KeywordsAdult, Aged, Biomarkers, CD4-Positive T-Lymphocytes, CD8-Positive T-Lymphocytes, Cell Separation, Cytokines, Disease Progression, Flow Cytometry, Humans, Inflammation Mediators, Ionomycin, Lung, Lymphocyte Activation, Middle Aged, Prognosis, Radiography, Sarcoidosis, Pulmonary, Tetradecanoylphorbol Acetate, Th1-Th2 Balance, Young Adult
Abstract

In pulmonary sarcoidosis, differential cytokine production in the lungs could be related to variable prognosis of patients at different stages of disease. Twenty patients with pulmonary sarcoidosis (10 at radiographic stage I and 10 at stages II-IV), as well as 10 age-matched healthy volunteers participated in the study. A 4-colour flow cytometric technique was used to measure interferon-γ (IFN-γ), interleukin (IL)-2, tumour necrosis factor-α (TNF-α), IL-4, and IL-13 production in phorbol myristate acetate (PMA)/ionomycin-stimulated CD4+ and CD8+ T cells from bronchoalveolar lavage fluid (BALF) and peripheral blood (PB) of patients, and PB of control subjects. CD4+ T cells from patients showed higher expression of IFN-γ in BALF than in PB. Significant correlations were observed between the percentages of BALF CD4+ and CD8+ T cells expressing intracellular IFN-γ, IL-2, and TNF-α. Stage I patients had lower percentages of IFN-γ-producing CD4+ and CD8+ T cells, as well as TNF-α-producing CD8+ T cells, in BALF (but not in PB) than stage II-IV patients. A decreased TH1 and TC1 response was demonstrated in BALF of patients at stage I of disease, which could explain their anticipated better prognosis.

DOI10.1089/jir.2012.0022
Alternate JournalJ Interferon Cytokine Res
PubMed ID23656599

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