Exercise intensity-dependent immunomodulatory effects on encephalomyelitis.
Title | Exercise intensity-dependent immunomodulatory effects on encephalomyelitis. |
Publication Type | Journal Article |
Year of Publication | 2019 |
Authors | Fainstein, N., Tyk R., Touloumi O., Lagoudaki R., Goldberg Y., Agranyoni O., Navon-Venezia S., Katz A., Grigoriadis N., Ben-Hur T., & Einstein O. |
Journal | Ann Clin Transl Neurol |
Volume | 6 |
Issue | 9 |
Pagination | 1647-1658 |
Date Published | 2019 09 |
ISSN | 2328-9503 |
Keywords | Animals, Chemokines, Cytokines, Encephalomyelitis, Autoimmune, Experimental, Gene Expression, Lymph Nodes, Lymphocyte Activation, Mice, Physical Conditioning, Animal, T-Lymphocytes |
Abstract | BACKGROUND: Exercise training (ET) has beneficial effects on multiple sclerosis and its animal model experimental autoimmune encephalomyelitis (EAE). However, the intensity-dependent effects of ET on the systemic immune system in EAE remain undefined.OBJECTIVE: (1) To compare the systemic immune modulatory effects of moderate versus high-intensity ET protocols in protecting against development of EAE; (2) To investigate whether ET affects autoimmunity selectively, or causes general immunosuppression.METHODS: Healthy mice performed moderate or high-intensity treadmill running programs. Proteolipid protein (PLP)-induced transfer EAE was utilized to examine ET effects specifically on the systemic immune system. Lymph node (LN)-T cells from trained versus sedentary donor mice were transferred to naïve recipients and EAE severity was assessed, by clinical assessment and histopathological analysis. LN-T cells derived from donor trained versus sedentary PLP-immunized mice were analyzed in vitro for proliferation assays by flow cytometry analysis and cytokine and chemokine receptor gene expression using real-time PCR. T cell-dependent immune responses of trained versus sedentary mice to the nonautoantigen ovalbumin and susceptibility to Escherichia coli-induced acute peritonitis were examined.RESULTS: High-intensity training in healthy donor mice induced significantly greater inhibition than moderate-intensity training on proliferation and generation of encephalitogenic T cells in response to PLP-immunization, and on EAE severity upon their transfer into recipient mice. High-intensity training also inhibited LN-T cell proliferation in response to ovalbumin immunization. E. coli bacterial counts and dissemination were not affected by training.INTERPRETATION: High-intensity training induces superior effects in preventing autoimmunity in EAE, but does not alter immune responses to E. coli infection. |
DOI | 10.1002/acn3.50859 |
Alternate Journal | Ann Clin Transl Neurol |
PubMed ID | 31368247 |
PubMed Central ID | PMC6764499 |
Grant List | / / The Judy and Sidney Swartz Fund for research in multiple sclerosis / International / / Chief Scientist Office of the Israeli Ministry of Health / International |