p16 promoter methylation in Pb2+ -exposed individuals.
| Title | p16 promoter methylation in Pb2+ -exposed individuals. |
| Publication Type | Journal Article |
| Year of Publication | 2010 |
| Authors | Kovatsi, L., Leda K., Georgiou E., Elisavet G., Ioannou A., Antrea I., Haitoglou C., Costas H., Tzimagiorgis G., George T., Tsoukali H., Helen T., Kouidou S., & Sofia K. |
| Journal | Clin Toxicol (Phila) |
| Volume | 48 |
| Issue | 2 |
| Pagination | 124-8 |
| Date Published | 2010 Feb |
| ISSN | 1556-9519 |
| Keywords | Adult, Case-Control Studies, CpG Islands, Cytosine, DNA Methylation, Genes, p16, Humans, Lead, Lead Poisoning, Nervous System, Adult, Male, Middle Aged, Occupational Exposure, Polymerase Chain Reaction, Promoter Regions, Genetic |
| Abstract | BACKGROUND: One of the principle symptoms of lead poisoning is the development of neurological disorders. Neuronal response is closely related to DNA methylation changes. Aim. In this study, we estimated p16 methylation in nine individuals exposed to lead using methylation-specific polymerase chain reaction followed by analysis of the methylated cytosine content of the product by thermal denaturation.RESULTS: We found that, based on lead blood concentration, lead-exposed individuals were divided into two groups. Among highly exposed individuals (blood Pb(2+) concentration = 51-100 microg/dL), we observed complete CpG methylation, whereas for low Pb(2+) concentrations (blood Pb(2+) concentration = 6-11 microg/dL), we observed partial methylation.CONCLUSION: Our results show that among lead-overexposed individuals, p16 methylation is frequent and extensive, and suggest that DNA methylation could be involved in the mechanism by which lead induces neurotoxicity. |
| DOI | 10.3109/15563650903567091 |
| Alternate Journal | Clin Toxicol (Phila) |
| PubMed ID | 20199129 |
