Breast tumor developed in a pregnant rat after treatment with the teratogen Cycloheximide.
Title | Breast tumor developed in a pregnant rat after treatment with the teratogen Cycloheximide. |
Publication Type | Journal Article |
Year of Publication | 2010 |
Authors | Emmanouil-Nikoloussi, E. N., Nikoloussis E., Manthou M. E., O Goula C., Likartsis C., Papamitsou T., Frangou H., Massouridou S., Lazaridis C., & Manthos A. |
Journal | Hippokratia |
Volume | 14 |
Issue | 2 |
Pagination | 136-8 |
Date Published | 2010 Apr |
ISSN | 1790-8019 |
Abstract | AIM: To describe histochemically and immunohistochemically a breast tumor presented in a pregnant rat given Cycloheximide to examine its teratogenic and embryotoxic effect on the embryos.METHODS: Cycloheximide was injected in pregnant Wistar rats at a dose of 3 mg/kg.b.w. on both 10th and 11th gestational days. In one of the rats, a large breast tumor developed rapidly. Histochemical staining with Hematoxylin-Eosin and Masson Trichrome and immunohistochemical identification with mouse monoclonal antibodies: a) Estrogen Receptor A and b) Estrogen Receptor B was performed.RESULTS: Analysis with A-receptor and B-receptor showed that the breast tumor which was developed after treatment with Cycloheximide was malignant. Positive immunohistochemical reaction was evident especially with A-receptor indicating the malignancy of the tumor.CONCLUSIONS: Cycloheximide is a known toxic and teratogenic agent and potentially a carcinogenic drug. Thus it should be used with extreme caution as a pesticide. |
Alternate Journal | Hippokratia |
PubMed ID | 20596273 |
PubMed Central ID | PMC2895279 |