Molecular and epidemiological characterization of HIV-1 infection networks involving transmitted drug resistance mutations in Northern Greece.
Title | Molecular and epidemiological characterization of HIV-1 infection networks involving transmitted drug resistance mutations in Northern Greece. |
Publication Type | Journal Article |
Year of Publication | 2011 |
Authors | Skoura, L., Metallidis S., Buckton A. J., Mbisa J. L., Pilalas D., Papadimitriou E., Papoutsi A., Haidich A-B., Chrysanthidis T., Tsachouridou O., Antoniadou Z. A., Kollaras P., Nikolaidis P., & Malisiovas N. |
Journal | J Antimicrob Chemother |
Volume | 66 |
Issue | 12 |
Pagination | 2831-7 |
Date Published | 2011 Dec |
ISSN | 1460-2091 |
Keywords | Adult, Anti-HIV Agents, Cluster Analysis, Drug Resistance, Viral, Female, Genotype, Greece, HIV Infections, HIV-1, Humans, Male, Middle Aged, Molecular Epidemiology, Mutation, Phylogeny, Prevalence |
Abstract | OBJECTIVES: To determine the contribution of transmission clusters to transmitted drug resistance (TDR) in newly diagnosed antiretroviral-naive HIV-1-infected patients in Northern Greece during 2000-07.METHODS: The prevalence of TDR was estimated in 369 individuals who were diagnosed with HIV-1 infection in the period 2000-07 at the National AIDS Reference Laboratory of Northern Greece. Phylogenetic analysis was performed using a maximum likelihood method on partial pol sequences. TDR was defined in accordance with the surveillance drug resistance mutation list (2009 update).RESULTS: The overall prevalence of TDR in our population was 12.5% [46/369, 95% confidence interval (CI) 9.1%-15.8%], comprising 7.6% (28/369) resistant to nucleoside reverse transcriptase inhibitors, 5.4% (20/369) resistant to non-nucleoside reverse transcriptase inhibitors and 3.3% (12/369) resistant to protease inhibitors. Dual class resistance was identified in 3.8% (14/369). Infection with subtype A was the sole predictor associated with TDR in multivariate analysis (odds ratio 2.15, 95% CI 1.10-4.19, P = 0.025). Phylogenetic analyses revealed three statistically robust transmission clusters involving drug-resistant strains, including one cluster of 12 patients, 10 of whom were infected with a strain carrying both T215 revertants and Y181C mutations.CONCLUSIONS: Our findings underline the substantial impact of transmission networks on TDR in our population. |
DOI | 10.1093/jac/dkr386 |
Alternate Journal | J. Antimicrob. Chemother. |
PubMed ID | 21933785 |