Clonidine and glucagon stimulation for testing growth hormone secretion in children and adolescents: can we make it with fewer samples?
Title | Clonidine and glucagon stimulation for testing growth hormone secretion in children and adolescents: can we make it with fewer samples? |
Publication Type | Journal Article |
Year of Publication | 2013 |
Authors | Christoforidis, A., Triantafyllou P., Slavakis A., & Katzos G. |
Journal | J Endocrinol Invest |
Volume | 36 |
Issue | 11 |
Pagination | 1046-50 |
Date Published | 2013 Dec |
ISSN | 1720-8386 |
Keywords | Adolescent, Child, Clonidine, False Positive Reactions, Female, Glucagon, Growth Disorders, Human Growth Hormone, Humans, Male, Retrospective Studies, Stimulation, Chemical |
Abstract | BACKGROUND: Our aim was to retrospectively evaluate the possibility to reduce the number of GH analyses during clonidine and glucagon tests without compromising accuracy.SUBJECTS AND METHODS: Two hundred and forty-five tests were performed in a total of 188 children and adolescents with a mean age of 9.93 ± 2.88 yr in a single center during the last 5 yr.RESULTS: Ninety-one out of 158 (57.59%) clonidine tests and 47/87 (54.02%) glucagon tests had at least one sample >10 μg/l (negative). For clonidine tests, not measuring GH at 30 min would have resulted in only one negative test missed (0.63% false positive result), whereas not measuring GH both at 0 and 30 min would have increased the false positive percentage to 2.53%. Ending clonidine tests at 90 min would have resulted in 7 negative tests missed (4.43% false positive results). For glucagon tests, more than half of the tests peaked at 120 min (56.32%). Skipping sampling at 0, 60 and 180 min provided a false positive rate of 5.75%.CONCLUSIONS: For clonidine tests we can omit blood sampling at time points 0 and 30 min without significantly compromising accuracy. |
DOI | 10.3275/9061 |
Alternate Journal | J. Endocrinol. Invest. |
PubMed ID | 23888314 |