The english version of the website is under development. Wherever text appears in Greek, it means it has not been translated yet.

Δημοσίευση

Effect of ropivacaine infiltration on muscle regeneration: a morphometric analysis.

TitleEffect of ropivacaine infiltration on muscle regeneration: a morphometric analysis.
Publication TypeJournal Article
Year of Publication2013
AuthorsKapoukranidou, D., Amaniti E., Kalpidis J., Karakoulas K., Papazisis G., Albani M., & Kouvelas D.
JournalHippokratia
Volume17
Issue4
Pagination351-4
Date Published2013 Oct
ISSN1108-4189
Abstract

BACKGROUND/AIM: Local anaesthetic myotoxicity is a well described phenomenon resulting in reversible muscle damage. Considering that in previous studies microscopic images were evaluated without quantification of morphologic characteristics, the aim of the present study was evaluate muscle regeneration after local anaesthetic infiltration.MATERIALS AND METHODS: Wistar rats underwent injection of the left tibialis anterior muscle with ropivacaine (0.75%, group HC or 0.375%, group LC), while the contralateral muscle was injected with saline (group SL). Six weeks later, the muscles were dissected, stained using acid ATPase and examined under light microscope coupled with a computer imaging system for morphometric analysis. Sections were evaluated regarding the content of different muscle fibre types (type I, IIa and IIb), fibre cross-section area and perimeter.RESULTS: Groups were comparable regarding the ratio of different muscle fibre types. Regenerated type I fibres of both HC and LC groups had significant greater mean cross-sectional area and perimeter, compared to SL fibres. No signs of necrosis or inflammation were observed. Type IIa and IIb fibres didn't show significant differences.CONCLUSIONS: Regenerated muscles, following local anaesthetic application, showed long-term morphological differences, which could lead to impaired function. Further studies are needed, in order to clarify the underlying cellular mechanisms and the subsequent possible functional impairment.

Alternate JournalHippokratia
PubMed ID25031515
PubMed Central IDPMC4097417

Contact

Secretariat of the School of Medicine
 

Connect

School of Medicine's presence in social networks
Follow Us or Connect with us.