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Detection of West Nile virus lineage 2 in the urine of acute human infections.

TitleDetection of West Nile virus lineage 2 in the urine of acute human infections.
Publication TypeJournal Article
Year of Publication2014
AuthorsPapa, A., Testa T., & Papadopoulou E.
JournalJ Med Virol
Volume86
Issue12
Pagination2142-5
Date Published2014 Dec
ISSN1096-9071
KeywordsAdult, Aged, Aged, 80 and over, Female, Greece, Humans, Male, Middle Aged, Molecular Diagnostic Techniques, Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction, RNA, Viral, Specimen Handling, Urine, West Nile Fever, West Nile virus, Young Adult
Abstract

West Nile virus (WNV) lineage 2 emerged in Greece in 2010 and since then outbreaks in humans have been reported for four consecutive years. Laboratory diagnosis is based mainly on serology. A real-time RT-PCR was applied on urine samples obtained from 35 patients with acute WNV infection. WNV RNA was detected in 40% of the samples with cycle threshold (CT) values ranging from 26.95 to 39.89 (mean 33.11). WNV was isolated from two of four urine samples with low CT (<30). Viral load was not associated with patients' age, sex, day of illness, presence of WNV antibodies, and neurological symptoms. However, it seems that sample shipment and storage conditions are very important for virus detection and isolation. The usefulness of the WNV RNA detection in urine as a diagnostic tool of acute WNV infections is discussed.

DOI10.1002/jmv.23949
Alternate JournalJ. Med. Virol.
PubMed ID24760617

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