The english version of the website is under development. Wherever text appears in Greek, it means it has not been translated yet.

Δημοσίευση

Key regulators control distinct transcriptional programmes in blood progenitor and mast cells.

TitleKey regulators control distinct transcriptional programmes in blood progenitor and mast cells.
Publication TypeJournal Article
Year of Publication2014
AuthorsCalero-Nieto, F. J., Ng F. S., Wilson N. K., Hannah R., Moignard V., Leal-Cervantes A. I., Jimenez-Madrid I., Diamanti E., Wernisch L., & Göttgens B.
JournalEMBO J
Volume33
Issue11
Pagination1212-26
Date Published2014 Jun 2
ISSN1460-2075
KeywordsAnimals, Cell Line, Gene Expression Profiling, Gene Expression Regulation, Genes, Reporter, Genome-Wide Association Study, Hematopoiesis, Mast Cells, Mice, Models, Statistical, Nucleotide Motifs, Oligonucleotide Array Sequence Analysis, Sequence Analysis, DNA, Sequence Analysis, RNA, Stem Cells, Transcription Factors, Transcription, Genetic
Abstract

Despite major advances in the generation of genome-wide binding maps, the mechanisms by which transcription factors (TFs) regulate cell type identity have remained largely obscure. Through comparative analysis of 10 key haematopoietic TFs in both mast cells and blood progenitors, we demonstrate that the largely cell type-specific binding profiles are not opportunistic, but instead contribute to cell type-specific transcriptional control, because (i) mathematical modelling of differential binding of shared TFs can explain differential gene expression, (ii) consensus binding sites are important for cell type-specific binding and (iii) knock-down of blood stem cell regulators in mast cells reveals mast cell-specific genes as direct targets. Finally, we show that the known mast cell regulators Mitf and c-fos likely contribute to the global reorganisation of TF binding profiles. Taken together therefore, our study elucidates how key regulatory TFs contribute to transcriptional programmes in several distinct mammalian cell types.

DOI10.1002/embj.201386825
Alternate JournalEMBO J.
PubMed ID24760698
PubMed Central IDPMC4168288
Grant ListBBS/B/1454X / / Biotechnology and Biological Sciences Research Council / United Kingdom
G0900951 / / Medical Research Council / United Kingdom
MC_U105260799 / / Medical Research Council / United Kingdom
/ / Wellcome Trust / United Kingdom

Contact

Secretariat of the School of Medicine
 

Connect

School of Medicine's presence in social networks
Follow Us or Connect with us.