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Detection of West Nile virus and insect-specific flavivirus RNA in Culex mosquitoes, central Macedonia, Greece.

TitleDetection of West Nile virus and insect-specific flavivirus RNA in Culex mosquitoes, central Macedonia, Greece.
Publication TypeJournal Article
Year of Publication2014
AuthorsPapa, A., Papadopoulou E., Kalaitzopoulou S., Tsioka K., & Mourelatos S.
JournalTrans R Soc Trop Med Hyg
Volume108
Issue9
Pagination555-9
Date Published2014 Sep
ISSN1878-3503
KeywordsAnimals, Culex, Databases, Nucleic Acid, Flavivirus, Genetic Variation, Greece, Humans, Phylogeny, Population Surveillance, Public Health, Reverse Transcriptase Polymerase Chain Reaction, RNA, Viral, West Nile Fever, West Nile virus
Abstract

BACKGROUND: West Nile virus (WNV) emerged in central Macedonia, northern Greece, in 2010. The aim of the study was to test Culex mosquitoes for genetic detection of WNV and other flaviviruses, and to check whether the detection of WNV in mosquitoes was correlated with the occurrence of human WNV cases.METHODS: During 2013, 25 780 Culex spp. mosquitoes were collected from central Macedonia, grouped into 295 pools, according to collection site and date, and tested for the presence of flavivirus RNA.RESULTS: Thirteen (4.4%) pools were flavivirus-positive. WNV lineage 2 was detected in nine (3.1%) pools, and insect-specific flaviviruses (ISFV) in four (1.4%). Three ISFV nucleotide sequences were 98% identical to Culex theileri flavivirus, previously detected in the Iberian peninsula, while the fourth sequence differed by >25% from all known flaviviruses. In most units of central Macedonia WNV detection in mosquitoes preceded the occurrence of human cases by approximately one month.CONCLUSIONS: Detection of WNV lineage 2 in mosquitoes for a fourth consecutive year suggests that the virus is established in Greece. Entomological surveillance provides an early warning system for the circulation of the virus. The detection of Culex theileri flavivirus extends the known range of this virus.

DOI10.1093/trstmh/tru100
Alternate JournalTrans. R. Soc. Trop. Med. Hyg.
PubMed ID25033823

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