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Virosome engineering of colloidal particles and surfaces: bioinspired fusion to supported lipid layers.

TitleVirosome engineering of colloidal particles and surfaces: bioinspired fusion to supported lipid layers.
Publication TypeJournal Article
Year of Publication2016
AuthorsFleddermann, J., Diamanti E., Azinas S., Košutić M., Dähne L., Estrela-Lopis I., Amacker M., Donath E., & Moya S. E.
JournalNanoscale
Volume8
Issue15
Pagination7933-41
Date Published2016 Apr 21
ISSN2040-3372
KeywordsBioengineering, Colloids, Hemagglutinin Glycoproteins, Influenza Virus, Humans, Hydrogen-Ion Concentration, Influenza A Virus, H1N1 Subtype, Membrane Fusion, Membrane Lipids, Membranes, Artificial, Microscopy, Atomic Force, Nanoparticles, Quartz Crystal Microbalance Techniques, Virosomes
Abstract

Immunostimulating reconstituted influenza virosomes (IRIVs) are liposomes with functional viral envelope glycoproteins: influenza virus hemagglutinin (HA) and neuraminidase intercalated in the phospholipid bilayer. Here we address the fusion of IRIVs to artificial supported lipid membranes assembled on polyelectrolyte multilayers on both colloidal particles and planar substrates. The R18 assay is used to prove the IRIV fusion in dependence of pH, temperature and HA concentration. IRIVs display a pH-dependent fusion mechanism, fusing at low pH in analogy to the influenza virus. The pH dependence is confirmed by the Quartz Crystal Microbalance technique. Atomic Force Microscopy imaging shows that at low pH virosomes are integrated in the supported membrane displaying flattened features and a reduced vertical thickness. Virosome fusion offers a new strategy for transferring biological functions on artificial supported membranes with potential applications in targeted delivery and sensing.

DOI10.1039/c5nr08169f
Alternate JournalNanoscale
PubMed ID27006101

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