Effect of ceftriaxone on the outcome of murine pyelonephritis caused by extended-spectrum-β-lactamase-producing Escherichia coli.
Τίτλος | Effect of ceftriaxone on the outcome of murine pyelonephritis caused by extended-spectrum-β-lactamase-producing Escherichia coli. |
Publication Type | Journal Article |
Year of Publication | 2014 |
Authors | Tratselas, A., Simitsopoulou M., Giannakopoulou A., Dori I., Saoulidis S., Kollios K., Papaioannidou P., Pournaras S., & Roilides E. |
Journal | Antimicrob Agents Chemother |
Volume | 58 |
Issue | 12 |
Pagination | 7102-11 |
Date Published | 2014 Dec |
ISSN | 1098-6596 |
Λέξεις κλειδιά | Animals, Anti-Bacterial Agents, Bacterial Load, beta-Lactamases, Ceftriaxone, Disease Models, Animal, Escherichia coli, Escherichia coli Infections, Female, Gene Expression, Interleukin-6, Kidney, Mice, Mice, Inbred BALB C, Pyelonephritis, Treatment Outcome |
Abstract | Urinary tract infections (UTIs) due to extended-spectrum-β-lactamase (ESBL)-producing Enterobacteriaceae in children are becoming more frequent, and they are commonly treated initially with a second- or third-generation cephalosporin. We developed a murine model of ascending UTI caused by ESBL-producing Escherichia coli. Using this model, we investigated the renal bacterial burden, interleukin-6 (IL-6) expression, and histopathological alterations caused by ESBL- and non-ESBL-producing bacteria after 1, 2, or 6 days with or without ceftriaxone therapy. The renal bacterial burden, IL-6 concentration, and histological inflammatory lesions were not significantly different between mice infected with ESBL- and non-ESBL-producing bacteria without treatment at any of the time points examined. Following ceftriaxone administration, the bacterial burden was eliminated in the kidneys of mice infected with ESBL- and non-ESBL-producing bacteria on the 6th postinfection day. The histological analysis demonstrated that among mice treated with ceftriaxone, those infected with ESBL-producing bacteria had more profound renal alterations than those infected with non-ESBL-producing bacteria on the 6th day (P < 0.001). In comparison, microbiological outcomes did not differ significantly between mice infected with ESBL- and non-ESBL-producing bacteria at any of the time points examined. The effectiveness of ceftriaxone in mice with UTIs due to ESBL-producing E. coli may have therapeutic implications; it is, however, hampered by limited activity on the histopathological lesions, a finding that needs further investigation. |
DOI | 10.1128/AAC.03974-14 |
Alternate Journal | Antimicrob. Agents Chemother. |
PubMed ID | 25224003 |
PubMed Central ID | PMC4249555 |