Increased monocyte alphaL, alphaM and beta2 integrin subunits in diabetes mellitus.
| Τίτλος | Increased monocyte alphaL, alphaM and beta2 integrin subunits in diabetes mellitus. |
| Publication Type | Journal Article |
| Year of Publication | 2009 |
| Authors | Kostidou, E., Koliakos G., & Kaloyianni M. |
| Journal | Clin Biochem |
| Volume | 42 |
| Issue | 7-8 |
| Pagination | 634-40 |
| Date Published | 2009 May |
| ISSN | 1873-2933 |
| Λέξεις κλειδιά | Aged, Antigens, CD11a, Antigens, CD11b, Antigens, CD18, Case-Control Studies, Diabetes Mellitus, Type 2, Female, Glucose, Humans, Insulin, Male, Middle Aged, Monocytes |
| Abstract | OBJECTIVES: Previous studies indicated the involvement of alpha2 (CD49b) integrin subunit in monocyte-laminin attachment in diabetes. The aim of the present study was to further investigate monocyte atherosclerosis related integrin subunit changes in relation to diabetes and hyperglycemia.DESIGN AND METHODS: Monocytes were derived from 10 patients with type II diabetes and 10 age matched controls. Attachment to laminin-1 in the presence of anti-integrin subunit antibodies has been estimated by a solid phase assay while for the alpha2 integrin subunit density assessment, fluorescence spectrometry was used.RESULTS: Incubation of monocytes with monoclonal anti-alphaL (CD11a) and anti-beta2 (CD18) equalized the differences between diabetics and controls. Both glucose and insulin increased the alpha2 integrin subunit expression in relation to the controls.CONCLUSIONS: The previously observed modified interaction between monocytes and laminin in diabetes mellitus may be attributed to changes not only in alpha2 but also in alphaL and beta2 integrin subunit changes. |
| DOI | 10.1016/j.clinbiochem.2008.12.007 |
| Alternate Journal | Clin. Biochem. |
| PubMed ID | 19135988 |
