In situ detection of hTERT variants in anaplastic large cell lymphoma.
Title | In situ detection of hTERT variants in anaplastic large cell lymphoma. |
Publication Type | Journal Article |
Year of Publication | 2006 |
Authors | Kotoula, V., Bobos M., Kostopoulos I., Kaloutsi V., Koletsa T., Karayannopoulou G., & Papadimitriou C. S. |
Journal | Leuk Lymphoma |
Volume | 47 |
Issue | 8 |
Pagination | 1639-50 |
Date Published | 2006 Aug |
ISSN | 1042-8194 |
Keywords | Adolescent, Adult, Aged, Female, Genetic Variation, Humans, Immunohistochemistry, In Situ Hybridization, Lymphocytes, Lymphoma, Large-Cell, Anaplastic, Male, Middle Aged, Neoplasm Proteins, RNA, Messenger, Telomerase |
Abstract | The expression of hTERT and its isoforms is difficult to assess in lymphoma tissues with the commonly used reverse transcription-polymerase chain reaction (RT-PCR) methods, because non-neoplastic lymphocytes expressing hTERT are always present in the lymphomatous infiltrates. The present study aimed to investigate hTERT mRNA variants in anaplastic large cell lymphoma (ALCL) (n = 38) with in situ hybridization (ISH), along with the immunodetection of hTERT protein. Probes for the identification of mRNAs containing (Bplus) and lacking (Bdel) exons 7 and 8 of the hTERT mRNA were used. Normal lymphocyte populations equally expressed both Bplus and Bdel mRNAs. Although all ALCL examined were found positive for hTERT expression with RT-PCR, hTERT mRNAs were identified in 68% of these tumors with ISH, with a higher incidence in the group bearing ALK translocations (10 out of 11; 90.9%) compared to the ALK negative group (17 out of 27; 59.3%) (PPearson's = 0.002). The same results were obtained with immunohistochemistry for hTERT. In approximately 50% of cases, only Bplus positive cells were identified, again with a higher incidence in the ALK positive compared to the ALK negative group (PPearson's = 0.016). In conclusion, ISH for hTERT mRNAs appears to be a valuable tool for the investigation of hTERT expression in lymphomas. Aberrations in hTERT variant profiles and a decline in the expression of the B deleted isoform may be associated with the pathogenesis of ALCL, especially with respect to ALK positive tumors. |
DOI | 10.1080/10428190600653317 |
Alternate Journal | Leuk Lymphoma |
PubMed ID | 16966278 |