The oxidative effect of prolonged CO(2) pneumoperitoneum a comparative study in rats.
Title | The oxidative effect of prolonged CO(2) pneumoperitoneum a comparative study in rats. |
Publication Type | Journal Article |
Year of Publication | 2012 |
Authors | Kontoulis, T. M., Pissas D. G., Pavlidis T. E., Pissas G. G., Lalountas M. A., Koliakos G., Topouridou K., & Sakantamis A. K. |
Journal | J Surg Res |
Volume | 175 |
Issue | 2 |
Pagination | 259-64 |
Date Published | 2012 Jun 15 |
ISSN | 1095-8673 |
Keywords | Animals, Biological Markers, Carbon Dioxide, Cytokines, Infusions, Parenteral, Intestine, Small, Kidney, Liver, Lung, Male, Malondialdehyde, Models, Animal, Oxidative Stress, Peroxidase, Pneumoperitoneum, Artificial, Rats, Rats, Wistar, Time Factors |
Abstract | BACKGROUND: The current study evaluated the effect of time in the severity of the oxidative stress due to pneumoperitoneum.METHODS: Forty Wistar rats were allocated randomly into 2 groups. The 1 h pneumoperitoneum (Pp) group, which was subjected to 60 min of pneumoperitoneum, and the 3 h Pp, to pneumoperitoneum for 180 min. The animals were divided in half. One half of the rats were left resting for 30 min after abdominal desufflation and the other for 8 h. After these two time periods, blood, liver, kidney, lung and small intestine were obtained for biochemical analysis and histopathological examination.RESULTS: In the 3 h Pp, the associated oxidative stress was increased. There was an overt increase in blood and tissue MDA and blood PAB values. The MPO values were significantly higher in the 3 h Pp group in serum, kidneys, and intestine during the early phase of reperfusion and in liver after 8 h of reperfusion. These changes occurred in the presence of light microscopic evidence of greater tissue damage for the 3 h Pp, which were consistent with the fluctuation of the MPO values.CONCLUSION: In our experimental model, we proved biochemically and histologically that time of maintenance of pneumoperitoneum is an additive factor that could cause increased oxidative stress in laparoscopic procedures. |
DOI | 10.1016/j.jss.2011.09.030 |
Alternate Journal | J. Surg. Res. |
PubMed ID | 22172128 |