Protein and mRNA expression of notch pathway components in operable tumors of patients with laryngeal cancer.
| Title | Protein and mRNA expression of notch pathway components in operable tumors of patients with laryngeal cancer. |
| Publication Type | Journal Article |
| Year of Publication | 2014 |
| Authors | Krikelis, D., Kotoula V., Bobos M., Fountzilas E., Markou K., Karasmanis I., Angouridakis N., Vlachtsis K., Kalogeras K. T., Nikolaou A., & Fountzilas G. |
| Journal | Anticancer Res |
| Volume | 34 |
| Issue | 11 |
| Pagination | 6495-503 |
| Date Published | 2014 Nov |
| ISSN | 1791-7530 |
| Keywords | Adult, Aged, Aged, 80 and over, Biomarkers, Tumor, Calcium-Binding Proteins, Carcinoma, Squamous Cell, Cell Nucleus, Cytoplasm, Female, Follow-Up Studies, Humans, Immunoenzyme Techniques, Intercellular Signaling Peptides and Proteins, Laryngeal Neoplasms, Male, Membrane Proteins, Middle Aged, Neoplasm Grading, Neoplasm Staging, Prognosis, Real-Time Polymerase Chain Reaction, Receptors, Notch, Reverse Transcriptase Polymerase Chain Reaction, RNA, Messenger, Survival Rate |
| Abstract | BACKGROUND: There exist substantial evidence that laryngeal cancer represents a unique entity among squamous head and neck carcinomas.MATERIALS AND METHODS: Tumors from 289 patients with squamous cell laryngeal cancer were assessed for protein (immunohistochemistry) and mRNA (qRT-PCR) expression of Notch pathway components (Notch1 to 4 receptors and Jagged1 ligand) on tissue microarrays.RESULTS: In univariate analysis, enhanced nuclear Jagged1 expression conferred a longer disease-free survival (DFS) (p=0.013) and overall survival (OS) (p=0.004), in contrast to the unfavorable prognostic value of Notch3 for both DFS (p=0.009) and OS (p=0.024). In multivariate analysis, overexpression of either Notch or cytoplasmic Jagged1 conferred an unfavorable effect on DFS (Hazard Ratio=1.88, 95% Confidence Interval=1.03-3.43, p=0.04).CONCLUSION: Our study indicates a consistent unfavorable effect of Notch3 and cytoplasmic Jagged1 protein expression, a favorable impact of nuclear Jagged 1 localization, and a differential prognostic value of Notch2 expression according to the presence of cytoplasmic Jagged 1. |
| Alternate Journal | Anticancer Res. |
| PubMed ID | 25368251 |
