Metabolic profile of human coelomic fluid.
| Title | Metabolic profile of human coelomic fluid. |
| Publication Type | Journal Article |
| Year of Publication | 2017 |
| Authors | Virgiliou, C., Valianou L., Witting M., Moritz F., Fotakis C., Zoumpoulakis P., Chatziioannou A. C., Lazaros L., Makrydimas G., Chatzimeletiou K., Raikos N., & Theodoridis G. A. |
| Journal | Bioanalysis |
| Volume | 9 |
| Issue | 1 |
| Pagination | 37-51 |
| Date Published | 2017 Jan |
| ISSN | 1757-6199 |
| Keywords | Body Fluids, Chromatography, Liquid, Embryo, Mammalian, Female, Gas Chromatography-Mass Spectrometry, Gestational Age, Gestational Sac, Humans, Magnetic Resonance Spectroscopy, Metabolome, Metabolomics, Tandem Mass Spectrometry |
| Abstract | AIM: Till now there is very limited knowledge on the molecular content of coelomic fluid and cells. This study presents the first attempt to elucidate the metabolic profile of such samples.METHODOLOGY: Samples were collected via coelocentesis from 41 women during the first trimester of gestation. Metabolic content was assessed using four different analytical platforms. For targeted analysis a hydrophilic interaction chromatography ultra high performance LC-MS/MS method was applied. Holistic analysis performed by GC-MS, NMR spectroscopy and ion cyclotron ultra-high resolution MS (FT-ICR-MS) instrumentation.RESULTS & CONCLUSIONS: Our observations suggest coelomic fluid and cells as promising biosamples, rich in metabolites with potential use in mammalian system biology studies. |
| DOI | 10.4155/bio-2016-0223 |
| Alternate Journal | Bioanalysis |
| PubMed ID | 27921458 |
